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4-1BB/CD137/TNFRSF9 (E2J5H) XP® Rabbit mAb (BSA and Azide Free)
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咨询Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to the extracellular domain of mouse 4-1BB/CD137/TNFRSF9 protein.
Product Usage Information
This product is the carrier-free version of product #18798. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Specificity/Sensitivity
Species Reactivity:
Mouse
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
参考图片
Flow cytometric analysis of live EL4 cells (blue) and CTLL-2 cells (green) using 4-1BB/CD137/TNFRSF9 (E2J5H) XP® Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Data were generated using the standard formulation of this product.
Western blot analysis of extracts from CTLL-2, EL4, and primary mouse CD8+ T cells, treated with anti-CD3 and anti-CD28 in the presence of mouse interleukin-2, using 4-1BB/CD137/TNFRSF9 (E2J5H) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Western blot analysis of extracts from mouse placenta and mouse skeletal muscle using 4-1BB/CD137/TNFRSF9 (E2J5H) XP® Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded mouse small intestine using 4-1BB/CD137/TNFRSF9 (E2J5H) XP® Rabbit mAb performed on the Leica® BOND™ Rx. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded 293T cell pellet, control (left, negative), mouse 4-1BB/CD137/TBFRSF9-transfected (middle, positive), and CTLL-2 cell pellet (right, positive) using 4-1BB/CD137/TNFRSF9 (E2J5H) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded 4T1 metastatic tumor in mouse lung using 4-1BB/CD137/TNFRSF9 (E2J5H) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded mouse thymus using 4-1BB/CD137/TNFRSF9 (E2J5H) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded mouse placenta using 4-1BB/CD137/TNFRSF9 (E2J5H) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded mouse spleen using 4-1BB/CD137/TNFRSF9 (E2J5H) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Confocal immunofluorescent analysis of mouse placenta using 4-1BB/CD137/TNFRSF9 (E2J5H) XP® Rabbit mAb (green). Actin filaments were labeled with Alexa Fluor® 647 Phalloidin #8940 (red). Samples were mounted in Prolong® Gold Antifade Reagent with DAPI #8961 (blue). Data were generated using the standard formulation of this product.
Confocal immunofluorescent analysis of CTLL-2 cells (left, positive) and EL4 cells (right, negative) using 4-1BB/CD137/TNFRSF9 (E2J5H) XP® Rabbit mAb (green). Actin filaments were labeled with Alexa Fluor® 647 Phalloidin (red) #8940. Blue = Hoechst 33342 #4082 (fluorescent DNA dye). Data were generated using the standard formulation of this product.