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TRAIL (C92B9) Rabbit mAb

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TRAIL (C92B9) Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

TRAIL

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys60 of human TRAIL, within the extracellular region of the protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunoprecipitation	1:50
Immunohistochemistry (Paraffin)	1:800
Immunofluorescence (Immunocytochemistry)	1:400
Flow Cytometry (Fixed/Permeabilized)	1:50
Flow Cytometry (Live)	1:50

同种型

Rabbit IgG

分子量

28-30

研究领域

癌症,细胞生物学,纤维化,神经科学,

应用

反应种属

Human

目标/特异性

Specificity/Sensitivity

TRAIL (C92B9) Rabbit mAb detects endogenous levels of total human TRAIL protein.

Species Reactivity:

Human

敏感性

Endogenous

背景

Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), also referred to as Apo2 ligand, first identified based on its sequence homology to TNF and Fas/Apo ligand is a member of the TNF family of cytokines and either exists as a type II membrane or soluble protein (1,2). TRAIL induces apoptosis in a variety of transformed cell lines and plays a role in anti-tumor and anti-viral immune surveillance (3). TRAIL signals via binding with death receptors DR4 (TRAIL-R1) (4) and DR5 (TRAIL-R2) (5-8) which can trigger apoptosis as well as NF-κB activation (7,9). Death domains on these receptors leads to the recruitment of a death-induced signaling complex (DISC) leading to caspase-8 and subsequent caspase-3 activation. In addition, TRAIL binds with decoy receptors DcR1 (TRAIL-R3) (6,8,10,11) and DcR2 (TRAIL-R4, TRUNDD) (12,13) which lack the functional cytoplasmic death domain antagonizing TRAIL-induced apoptosis. Osteoprotegerin (OPG) has also been identified as receptor capable of inhibiting TRAIL-induced apoptosis (14). The selectivity of soluble TRAIL at triggering apoptosis in transformed cells as compared to normal cells has led to its investigation as a potential cancer therapeutic (15,16). 1.Wiley, S.R. et al. (1995) Immunity 3, 673-82. 2.Pitti, R.M. et al. (1996) J Biol Chem 271, 12687-90. 3.Almasan, A. and Ashkenazi, A. Cytokine Growth Factor Rev 14, 337-48. 4.Pan, G. et al. (1997) Science 276, 111-3. 5.Walczak, H. et al. (1997) EMBO J 16, 5386-97. 6.MacFarlane, M. et al. (1997) J Biol Chem 272, 25417-20. 7.Chaudhary, P.M. et al. (1997) Immunity 7, 821-30. 8.Schneider, P. et al. (1997) FEBS Lett 416, 329-34. 9.Shetty, S. et al. (2002) Apoptosis 7, 413-20. 10.Sheridan, J.P. et al. (1997) Science 277, 818-21. 11.Degli-Esposti, M.A. et al. (1997) J Exp Med 186, 1165-70. 12.Pan, G. et al. (1998) FEBS Lett 424, 41-5. 13.Marsters, S.A. et al. (1997) Curr Biol 7, 1003-6. 14.Kelley, S.K. et al. (2001) J Pharmacol Exp Ther 299, 31-8. 15.Walczak, H. et al. (1999) Nat Med 5, 157-63. 16.Ashkenazi, A. et al. (1999) J Clin Invest 104, 155-62.

研究领域

癌症,细胞生物学,纤维化,神经科学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #48318.

数据库链接

Entrez-Gene ID

8743

UniProt ID

P50591

参考图片

Flow cytometric analysis of live THP-1 cells treated with TPA (80 nM, 18 h), rested for 48 h in fresh media, and then untreated (blue, negative) or treated with LPS (5 μg/mL, 18 h) (green, positive) and preincubated with human Fc block (2.5 μg/million cells, 10 min) using TRAIL (C92B9) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')₂ Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western blot analysis of extract from 786-0 cells and differentiated THP-1 cells, untreated or treated overnight with LPS, using TRAIL (C92B9) Rabbit mAb.

Western blot analysis of extracts from HeLa cells, mock transfected or transfected with human TRAIL expression construct, using TRAIL (C92B9) Rabbit mAb.

Simple Western™ analysis of 786-O (0.1 mg/mL) cell lysate using TRAIL (C92B9) Rabbit mAb #3219. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using TRAIL (C92B9) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lymphoma using TRAIL (C92B9) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded 786-0 (positive, left) or HeLa (negative, right) cell pellets using TRAIL (C92B9) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human tonsil using TRAIL (C92B9) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Confocal immunofluorescent analysis of 786-0 cells using TRAIL (C92B9) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Flow cytometric analysis of 786-0 cells using TRAIL (C92B9) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

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货号：

3219S

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20μl

100ul

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