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Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding cysteine 57 of human TRAF1.
Product Usage Information
This product is the carrier free version of product #4715. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Specificity/Sensitivity
Species Reactivity:
Human
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
参考图片
Flow cytometric analysis of Raji cells, using TRAF1 (45D3) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). Data were generated using the standard formulation of this product.
Western blot analysis of extracts from Raji and SR cells, using TRAF1 (45D3) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded human tonsil using TRAF1 (45D3) Rabbit mAb preincubated with a control peptide (left) or TRAF1 Blocking Peptide #1066 (right). Data were generated using the standard formulation of this product.
Confocal immunofluorescent analysis of HT-1080 (upper) and HEK/293 (lower) cells, untreated (left) or treated with TNF-α #8902 (right), using TRAF1 (45D3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Data were generated using the standard formulation of this product.