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TRIM33 (D7U4F) Rabbit mAb

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TRIM33 (D7U4F) Rabbit mAb

品牌： CST

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分子量：

反应种属：

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

TRIM33

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln609 of human TRIM33 protein.

宿主

Rabbit

商品描述

Product Usage Information

For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:200
Immunohistochemistry (Paraffin)	1:1000
Flow Cytometry (Fixed/Permeabilized)	1:200
Chromatin IP	1:50

同种型

Rabbit IgG

分子量

150

研究领域

癌症,发育生物学与干细胞研究,表观遗传学,神经科学,

应用

反应种属

Human

目标/特异性

Specificity/Sensitivity

TRIM33 (D7U4F) Rabbit mAb recognizes endogenous levels of total TRIM33 protein.

Species Reactivity:

Human

敏感性

Endogenous

背景

The transcriptional intermediary factor 1 (TIF1) family represents a group of proteins with multiple histone-binding domains. In humans, this family comprises four proteins, TIF1α/TRIM24, TIF1β/TRIM28/KAP1, TIF1γ/TRIM33/Ectodermin, and TIF1δ/TRIM66, which are characterized by an amino-terminal tripartite motif (TRIM) domain consisting of a RING domain, two B boxes, a coiled-coil domain, and a carboxy-terminal PHD finger and bromodomain (1). Despite their similar overall structure, these proteins have diverse roles in transcriptional regulation. TIF1α functions as a ligand-dependent nuclear receptor coregulator and more recently has been implicated in regulating p53 stability (2). TIF1β is an intrinsic component of the N-CoR1 corepressor complex and the NuRD nucleosome-remodeling complex (3) and functions as a corepressor for Kruppel-associated box (KRAB) zinc-finger transcription factors (4). Furthermore, TIF1β promotes heterochromatin-mediated gene silencing formation by serving as a cofactor for heterochromatin protein HP1 (5). TIF1δ expression is restricted to the testis and has been shown to interact with HP1γ (6). 1.Meroni, G. and Diez-Roux, G. (2005) Bioessays 27, 1147-57. 2.Jain, A.K. and Barton, M.C. (2009) Cell Cycle 8, 3668-74. 3.Underhill, C. et al. (2000) J Biol Chem 275, 40463-70. 4.Schultz, D.C. et al. (2001) Genes Dev 15, 428-43. 5.Groner, A.C. et al. (2010) PLoS Genet 6, e1000869. 6.Khetchoumian, K. et al. (2004) J Biol Chem 279, 48329-41.

研究领域

癌症,发育生物学与干细胞研究,表观遗传学,神经科学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

51592

UniProt ID

Q9UPN9

参考图片

Chromatin immunoprecipitations were performed with cross-linked chromatin from RKO cells, treated with JQ1 (1 μM, 3 hr) and either TRIM33 (D7U4F) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using Human c-Myc Exon 1 Primers, SimpleChIP® Human TGFBR2 Promoter Primers #39833, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Western blot analysis of extracts from various cell lines using TRIM33 (D7U4F) Rabbit mAb.

Immunoprecipitation of TRIM33 from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is TRIM33 (D7U4F) Rabbit mAb. Western blot analysis was performed using TRIM33 (D7U4F) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded MCF7 cell pellet (left, high-expressing) or U2OS cell pellet (right, low-expressing) using TRIM33 (D7U4F) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human infiltrating papillary carcinoma of the breast using TRIM33 (D7U4F) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using TRIM33 (D7U4F) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human clear cell adenocarcinoma of the ovary using TRIM33 (D7U4F) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using TRIM33 (D7U4F) Rabbit mAb.

Flow cytometric analysis of MCF7 cells using TRIM33 (D7U4F) Rabbit mAb (solid line) compared to a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

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货号：

90051S

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询价

供应商现货

20μl

100ul

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