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TRKB (EXTRACELLULAR) ANTIBODY
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产品详细信息
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).
靶标信息
NTRK2 (TRKB) belongs to the neurotrophic factor family of related polypeptides central to the development and maintenance of the mammalian nervous system. NTRK2 is the receptor for brain-derived neurotrophic factor (BDNF). Together, NTRK2 and BDNF regulate both short-term synaptic functions and long-term potentiation of brain synapses. TrkB is involved in the development and maintenance of the nervous system. Moreover, Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems. The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of Trk B gp145, one of the receptors encoded by Trk B, although BDNF elicits a response at least two orders of magnitude greater than NT-3. TrkB is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signaling through TrkB leads to cell differentiation. Mutations in the TrkB gene have been associated with obesity and mood disorders. Alternate transcriptional splice variants encoding different isoforms have been found for the TrkB gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: AI848316; BDNF-tropomyosine receptor kinase B; BDNF/NT-3 growth factors receptor; C030027L06Rik; EC 2.7.10.1; GP145 TrkB; GP145-TrkB; GP145-TrkB/GP95-TrkB; GP145TrkB; GP95 TrkB; GP95-TrkB; hyperphagia; kinase TrkB; neural receptor protein-tyrosine kinase (trkB); Neurotrophic tyrosine kinase receptor type 2; neurotrophic tyrosine kinase, receptor, type 2; neurotrophic tyrosine receptor kinase type 2; NTRK 2; OTTHUMP00000021573; OTTHUMP00000021574; OTTHUMP00000021576; OTTHUMP00000021577; Trk B; Trk-B; TrkB tyrosine kinase; Tropomyosin-related kinase B; tyrosine kinase receptor B
基因别名: GP145-TrkB; GP145-TrkB/GP95-TrkB; NTRK2; RATTRKB1; Tkrb; trk-B; TRKB; TRKB1
UniProt ID:(Human) Q16620, (Mouse) P15209, (Rat) Q63604
Entrez Gene ID:(Human) 4915, (Mouse) 18212, (Rat) 25054
参考图片
Expression of TrkB in rat PC12 cells - Cell surface detection of TrkB in live intact rat pheochromocytoma PC12 cells. A. Extracellular staining of cells with Anti-TrkB (extracellular) Antibody (#ANT-019) (1:50), followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Live view of the cells. C. Merge of the two images.
Expression of TrkB in rat DRG - Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-TrkB (extracellular) Antibody (#ANT-019), (1:100). TrkB (red) is expressed in DRG neurons. Hoechst 33342 is used as the counterstain.
Western blot analysis of rat brain membranes (lanes 1 and 3) and HEK-TrkB transfected cell lysates (lanes 2 and 4): - 1,2. Anti-TrkB (extracellular) Antibody (#ANT-019), (1:200).3,4. Anti-TrkB (extracellular) Antibody , preincubated with TrkB (extracellular) Blocking Peptide (#BLP-NT019).
Western blot analysis of mouse brain lysates: - 1. Anti-TrkB (extracellular) Antibody (#ANT-019), (1:200). 2. Anti-TrkB (extracellular) Antibody , preincubated with TrkB (extracellular) Blocking Peptide (#BLP-NT019).
Cell surface detection ofTrkBby indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells+ goat- Anti-rabbit-FITC. (green) Cells + Anti-TrkB (extracellular)&(8)239; Antibody &(8)239; (#ANT-019), (2.5μg) + goat- Anti-rabbit-FITC.