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咨询The TrueBlack® Fluorescent Western Blot Blocking Buffer Kit is specifically formulated to increase the specificity and sensitivity of the fluorescent western blot assay by blocking non-specific interaction between fluorophore-labeled antibodies and the western blotting membrane. This kit provides the reagents needed to block non-specific protein binding and background caused by charged dyes. The reagents in the kit are compatible with both nitrocellulose and polyvinylidene difluoride (PVDF) membranes. This kit is not intended to be used for chemiluminescence detection.
Store at 4ºC. A slight precipitate may be present, but will not interfere with product performance. All components in this kit are stable for 24 months when stored at 4ºC. Recommend warming to room temperature and mixing before use.
参考图片
Western blot analysis of extracts from Jurkat cells, untreated (-) or treated with LY294002 #9901 or Calyculin A #9902 (+), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (upper), Akt (pan) (C67E7) Rabbit mAb #4691 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower). The western blot membranes were treated with our standard antibody diluent and blocking buffers (left) or with the TrueBlack® Fluorescent Western Blot Blocking Buffer Kit (right). Increased antibody sensitivity and decreased background fluorescence can be seen when using the TrueBlack® Fluorescent Western Blot Blocking Buffer Kit.