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产品介绍
产品信息
抗原名称
beta-Tubulin
来源纯化
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human β-tubulin protein.
宿主
Rabbit
简单描述
Monoclonal Antibody for studying tubulin beta-5. Cited in 54 publications. Validated for WB, IHC, IF. Highly specific and rigorously validated in-house, β-Tubulin (D2N5G) Rabbit Monoclonal Antibody (CST #15115) is ready to ship.
商品描述
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:400 |
| Immunofluorescence (Immunocytochemistry) | 1:100 |
分子量
55
研究领域
癌症,细胞生物学,纤维化,神经科学,
应用
反应种属
Human,Mouse,Rat,Monkey,
目标/特异性
Specificity/Sensitivity
β-Tubulin (D2N5G) Rabbit mAb recognizes endogenous levels of total β-tubulin protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
敏感性
Endogenous
背景
背景
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
1.Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.
研究领域
癌症,细胞生物学,纤维化,神经科学,
翻译后修饰
Unmodified
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
数据库链接
Entrez-Gene ID
203068
UniProt ID
P07437
参考图片
Confocal immunofluorescent analysis of NIH/3T3 cells using β-Tubulin (D2N5G) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using β-Tubulin (D2N5G) Rabbit mAb.
Western blot analysis of extracts from HeLa, NIH/3T3, and KNRK cells using β-Tubulin (D2N5G) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using β-Tubulin (D2N5G) Rabbit mAb.
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