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Ubiquitin conjugating kit (HeLa lysate-based)
品牌： Enzo Life
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对比
咨询实验应用：
Uses:
1. Ubiquitinylation of specific endogenous HeLa lysate proteins, followed by their immediate detection/analysis using antibodies to the protein/s of interest, indicating a particular protein is a substrate for the ubiquitin-proteasome pathway.
2. Ubiquitinylation of proteins of interest contained in exogenously added expre
产品介绍
产品信息
试剂盒组分
HeLa S100 fraction (Prod. No. BML-SW8750), 1 mg (200µl provided as 2×100μl vials), 5mg/ml
10x Ubiquitin solution (UB) (Prod. No. BML-UW8795), 100µl (provided as 2X50μl vials), 1mg/ml
10x ATP-(Energy) regeneration solutions (ATP-ERS) (Prod. No. BML-EW9810), 100µl
10x Ubiquitinylation buffer (Prod. No. BML-KW9885), 100µl
Ubiquitin aldehyde (inhibitor) (Prod. No. BML-UW8450), 50 µl, 1mg/ml
Ubiquitin-conjugate specific antibody solution (UBCJ2) (Prod. No. ENZ-ABS840), 10 µl, Mono- and polyubiquitinylated conjugates, mAb
应用
实验应用
Uses:
1. Ubiquitinylation of specific endogenous HeLa lysate proteins, followed by their immediate detection/analysis using antibodies to the protein/s of interest, indicating a particular protein is a substrate for the ubiquitin-proteasome pathway.
2. Ubiquitinylation of proteins of interest contained in exogenously added expre
制备和贮存
保存
All kit components should be stored at -80°C to ensure stability and activity. Inhibitors are stable for up to 3 months at -20°C when dissolved in DMSO.
保存方式
-80°C
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