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Ubiquityl-Histone H2A (Lys119) (D27C4) XP ®  Rabbit mAb (BSA and Azide Free)
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Ubiquityl-Histone H2A (Lys119) (D27C4) XP ® Rabbit mAb (BSA and Azide Free)

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品牌: CST
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分子量:
23
来源宿主:
Rabbit
产品介绍
产品介绍
产品信息
荧光素标记
来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human histone H2A protein in which Lys119 is mono-ubiquitinated.

宿主
Rabbit
商品描述

Product Usage Information

This product is the carrier free version of product #8240. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.


This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.


BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

同种型
Rabbit IgG
分子量
23
应用
目标/特异性

Specificity/Sensitivity

Ubiquityl-Histone H2A (Lys119) (D27C4) XP Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of histone H2A protein only when ubiquitinated at Lys119. The antibody does not cross-react with other ubiquitinated proteins or free ubiquitin.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性
Endogenous
背景
背景
The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of posttranslational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1). Ubiquitin is a conserved 76 amino acid peptide unit that can be covalently linked to many cellular proteins by the ubiquitination process. Three components are involved in this protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thioester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (2). Histone H2A is mono-ubiquitinated at Lys119 by the Polycomb Repressor Complex 1 (PRC1) and is critical for transcriptional silencing of the developmental HOX genes and X chromosome inactivation (3-6). PRC1 is composed of Bmi1 and RING1A (also RING1 or RNF1), both of which act to enhance the E3 ubiquitin ligase activity of the catalytic subunit RING1B (also RING2 or RNF2) (3,4). Histone H2A is also mono-ubiquitinated at Lys119 at sites of DNA damage. This mono-ubiquitination event requires the PRC1 components Bmi1 and RING1B, in addition to another E3 ubiquitin ligase RNF8, and contributes to subsequent recruitment of the BRCA1 complex, via binding of RAP80/UIMC1 (ubiquitin interactive motif containing 1 protein) (7-10). 1.Peterson, C.L. and Laniel, M.A. (2004) Curr Biol 14, R546-51. 2.Liu, F. and Walters, K.J. (2010) Trends Biochem Sci 35, 352-60. 3.Wang, H. et al. (2004) Nature 431, 873-8. 4.Cao, R. et al. (2005) Mol Cell 20, 845-54. 5.de Napoles, M. et al. (2004) Dev Cell 7, 663-76. 6.Fang, J. et al. (2004) J Biol Chem 279, 52812-5. 7.Ginjala, V. et al. (2011) Mol Cell Biol 31, 1972-82. 8.Bergink, S. et al. (2006) Genes Dev 20, 1343-52. 9.Marteijn, J.A. et al. (2009) J Cell Biol 186, 835-47. 10.Wu, J. et al. (2009) Mol Cell Biol 29, 849-60.
翻译后修饰
ubiquitinate
制备和贮存
保存方式

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

数据库链接
Entrez-Gene ID
221613
UniProt ID
Q96QV6

参考图片

Flow cytometric analysis of HeLa cells using Ubiquityl-Histone H2A (Lys119) (D27C4) XP® Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Data were generated using the standard formulation of this product.

Western blot analysis of extracts from various cell lines using Ubiquityl-Histone H2A (Lys119) (D27C4) XP® Rabbit mAb. Data were generated using the standard formulation of this product.

Confocal immunofluorescent analysis of HeLa cells using Ubiquityl-Histone H2A (Lys119) (D27C4) XP® Rabbit mAb (green) and COX IV (4D11-B3-E8) Mouse mAb #11967 (red). Data were generated using the standard formulation of this product.

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货号:
45772SF
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询价
100μg
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