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Monoclonal antibody is produced by immunizing animals with a synthetic V5-tag peptide.
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:500 - 1:2000 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 - 1:1600 |
Specificity/Sensitivity
Species Reactivity:
All Species Expected
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #58009.
参考图片
Flow cytometric analysis of 293T cells, mock transfected (blue) or transfected with a V5-tagged construct (green), using V5-Tag (D3H8Q) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected with V5-tag fusion protein (+), using V5-Tag (D3H8Q) Rabbit mAb and β-Actin (D6A8) Rabbit mAb #8457.
Simple Western™ analysis of lysates (0.1 mg/mL) from COS-7 +V5-N-Ras cells using V5-Tag (D3H8Q) Rabbit mAb #13202. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Immunoprecipitation of transfected V5-tag fusion protein from COS-7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or V5-Tag (D3H8Q) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using V5-Tag (D3H8Q) Rabbit mAb.
Confocal immunofluorescent analysis of COS-7 cells, transfected with a V5-tagged construct (left) or mock transfected (right), using V5-Tag (D3H8Q) Rabbit mAb (green), DyLight™ 554 Phalloidin #13054 (red), and DRAQ5® #4084 (blue pseudocolor).