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V5-Tag (E9H8O) Mouse mAb (Sepharose Bead Conjugate)
品牌： CST
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对比
咨询反应种属：
All
来源宿主：
Mouse
产品介绍
产品介绍
产品信息
荧光素标记
Sepharose Bead
抗原名称
V5-Tag
来源纯化
Monoclonal antibody is produced by immunizing animals with a synthetic V5-tag peptide.
宿主
Mouse
简单描述
This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose beads. V5-Tag (E9H8O) Mouse mAb (Sepharose Bead Conjugate) is useful for immunoprecipitation assays. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated V5-Tag (E9H8O) Mouse mAb #80076.
商品描述
Product Usage Information
| Application | Dilution |
|---|---|
| Immunoprecipitation | 1:20 |
同种型
Mouse IgG2a kappa
应用
反应种属
All
目标/特异性
Specificity/Sensitivity
V5-Tag (E9H8O) Mouse mAb (Sepharose Bead Conjugate) recognizes transfected levels of recombinant protein containing the V5 epitope tag. This antibody also recognizes a non-specific band of unknown origin at 21 kDa.
Species Reactivity:
All Species Expected
敏感性
Transfected Only
背景
背景
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The V5-tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family (1). The V5-tag can be used to detect expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems.
1.Hanke, T. et al. (1995) J Virol Methods 53, 149-56.
翻译后修饰
unmodified
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibody.
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