Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb

Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8). 1.Meyer, M. et al. (1999) EMBO J 18, 363-74. 2.Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38. 3.Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7. 4.Takahashi, T. et al. (2001) EMBO J 20, 2768-78. 5.Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75. 6.Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605. 7.Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92. 8.Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.