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Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb

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Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb

品牌： CST

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参数

分子量：

230

反应种属：

Human,Mouse,

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产品介绍

产品信息

抗原名称

VEGF Receptor 2

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1175 of human VEGF receptor-2.

宿主

Rabbit

简单描述

Monoclonal Antibody for studying VEGFR2 (Tyr1175) phosphate. Cited in 346 publications. Validated for Western Blotting. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit Monoclonal Antibody (CST #2478) is ready to ship.

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000

分子量

230

研究领域

癌症,细胞生物学,

应用

反应种属

Human,Mouse,

目标/特异性

Specificity/Sensitivity

Phospho-VEGF Receptor-2 (Tyr1175) (19A10) Rabbit mAb detects endogenous levels of VEGFR-2 proteins only when phosphorylated at tyrosine 1175. This antibody may cross-react with VEGFR1.

Species Reactivity:

Human, Mouse

敏感性

Endogenous

背景

Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8). 1.Meyer, M. et al. (1999) EMBO J 18, 363-74. 2.Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38. 3.Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7. 4.Takahashi, T. et al. (2001) EMBO J 20, 2768-78. 5.Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75. 6.Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605. 7.Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92. 8.Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.

研究领域

癌症,细胞生物学,

翻译后修饰

Phosphate

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

3791

UniProt ID

P35968

参考图片

Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb specifically binds to phosphorylated VEGFR2, but not other phosphorylated tyrosine kinases. Western blot analysis of extracts from cells expressing different activated tyrosine kinase proteins, using Phospho-VEGF Receptor-2 (Tyr1175) (19A10) Rabbit mAb (upper) and Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower). CKR/PAE cells (lanes 12 and 13) express chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGF receptor-2 intracellular domain (7). CSF-1 stimulates phosphorylation of Tyr1175 of intracellular VEGF receptor-2 domain (lane 12), which was specifically detected by Phospho-VEGF Receptor-2 (Tyr1175) (19A10) Rabbit mAb. Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb 特异结合磷酸化的VEGFR2, 但不与其它磷酸化的酪氨酸激酶反应。用Phospho-VEGF Receptor-2 (Tyr1175) (19A10) Rabbit mAb (上)和 Phospho-Tyrosine mAb (P-Tyr-100) #9411 (下)对表达不同的活化的酪氨酸激酶蛋白的细胞提取物进行免疫印迹检测。CKR/PAE 细胞 (第12 和13道) 表达含有人的CSF-1胞外结合区/小鼠VEGF receptor 2胞内区的嵌合受体(7)。Phospho-VEGF Receptor-2 (Tyr1175) (19A10) Rabbit mAb 能特意地检测CSF-1刺激下VEGF receptor-2胞内区的Tyr1175 发生磷酸化 (第12道)。

Immunohistochemical analysis of paraffin-embedded HUVE cells, untreated (left) or VEGF treated (right). 对未经处理的（左）或经过VEGF处理的（右）石蜡包埋的HUVE细胞进行免疫组化检测。

Western blot analysis of extracts from HUVE cells, untreated or stimulated with VEGF (50 ng/ml for 2 minutes), using Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb (upper) and VEGF Receptor 2 (55B11) Rabbit mAb #2479 (lower). 用Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb (上)和 VEGF Receptor 2 (55B11) Rabbit mAb #2479 (下)对未经处理的或经过VEGF (50 ng/ml 处理2 minutes)的HUVE细胞的提取物进行免疫印迹检测。

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货号：

2478T

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