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产品信息
宿主
亚美尼亚仓鼠
克隆号
13F3
克隆类别
单抗
研究领域
免疫学和肿瘤学,癌症
Product Details
The 13F3 monoclonal antibody reacts with mouse V-domain Ig suppressor of T cell activation (VISTA) also known as PD-1H and B7-H5. VISTA is a 309 aa type I transmembrane glycoprotein and a member of the Ig superfamily. VISTA is expressed on naïve and activated T cells, NK cells, macrophages, dendritic cells, and neutrophils. VISTA functions as a negative immune-checkpoint protein that suppresses T cell cytokine production and proliferation. VISTA is overexpressed by tumor-infiltrating lymphocytes, such as myeloid cells and regulatory T cells. Blockade of VISTA with the 13F3 antibody results in delayed tumor growth in mouse models of melanoma.Specifications
| Isotype | Armenian hamster IgG |
|---|---|
| Recommended Isotype Control(s) | InVivoMAb polyclonal Armenian hamster IgG |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | EL4 cells overexpressing mouse VISTA-RFP and then boosted with VISTA-Ig fusion protein |
| Reported Applications | in vivo blocking of VISTA signaling in vitro blocking of VISTA signaling Flow cytometry |
| Formulation | PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin | <2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay |
| Purity | >95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| RRID | AB_2736990 |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
应用
反应种属
mouse
背景
研究领域
Cancer Biology, Immune Checkpoint Protein, Immunology
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