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hWIF-1 MAb (Cl 13301 (25 ug)

品牌： R&D Systems

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来源宿主：

Mouse IgG1

实验应用：

Western Blot, Immunohistochemistry

产品介绍

产品信息

耦联标记

Unconjugated

纯化方式

Protein A or G purified from hybridoma culture supernatant

抗原名称

WIF1， WIF-1， WNT inhibitory factor 1

宿主

Mouse IgG1

免疫原

Mouse myeloma cell line NS0-derived recombinant human WIF-1
Gly29-Trp379
Accession # AAD25402

克隆号

133015

Scientific Data

Immunohistochemistry

View Larger

WIF‑1 in Human Breast. WIF‑1 was detected in immersion fixed paraffin-embedded sections of human breast using 8 µg/mL Mouse Anti-Human WIF‑1 Monoclonal Antibody (Catalog # MAB134) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Human WIF-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human WIF-1 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse WIF‑1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 133015

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human WIF-1
Gly29-Trp379
Accession # AAD25402

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human WIF‑1 (Catalog # 1341-WF)

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

应用

实验应用

Western Blot, Immunohistochemistry

背景

别名

WIF1,WIF-1,WNT inhibitory factor 1,Wnt Inhibitory Factor

背景

Background: WIF-1

WIF-1 is a secreted protein that binds Wnt proteins and antagonizes Wnt-induced signaling. WIF-1 expression is down regulated in several human cancers.

Long Name

Wnt Inhibitory Factor

Entrez Gene IDs

11197 (Human); 24117 (Mouse)

Alternate Names

WIF1; WIF-1; WNT inhibitory factor 1

研究领域

Related Research Areas

制备和贮存

溶解方法

Reconstitute at 0.5 mg/mL in sterile PBS.

保存方式

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

参考图片

WIF‑1 in Human Breast. WIF‑1 was detected in immersion fixed paraffin-embedded sections of human breast using 8 µg/mL Mouse Anti-Human WIF‑1 Monoclonal Antibody (Catalog # MAB134) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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货号：

MAB134-SP

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询价

25ug

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