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Lys177-His266
57.5kDa (Reducing)
1μg (R: reducing condition, N: non-reducing condition).
Wnt surrogate protein is an engineered Fc fusion protein, which activates similar Wnt signaling pathways as most commercially available recombinant Wnt3a proteins or Wnt-conditioned media, while requiring a fraction of the amount of protein or hassle. It is necessary for cell proliferation and differentiation and the self-renewal of stem cells and is widely used for supporting organoid culture, including intestinal, stomach, liver, lung organoids. Modulation of WNT signaling pathways has potential for treatment of degenerative diseases and tissue injuries. This engineered Wnt surrogate protein can be selective for individual FZD or a subfamily of FZDs in combination with LRP5 or LRP6, depending on the binder specificities, and may be used in any of a variety of therapeutic methods for the treatment of diseases.
Recombinant Human Wnt3a Surrogate Protein activates Wnt signaling pathways and is necessary for cell proliferation and differentiation and the self-renewal of stem cells. It is widely used for supporting organoid culture, including intestinal, stomach, liver, lung organoids.
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Obrecht-Sturm, Schömig, Mynarek et al Neuro Oncol (2024)
Willert, K. and Nusse, R. (2012) Cold Spring Harb. Perspect. Biol. 4:a007864
Chen, Lee, Ouyang et al Antib Ther (2024) 7 (1), 88-95
参考图片
1μg (R: reducing condition, N: non-reducing condition).
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293T human embryonic kidney cells. The EC50 for this effect is less than 90ng/ml.