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咨询Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3006-WN
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
3006-WN/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Recombinant Mouse Wnt-5b Protein Summary
Product Specifications
Optimal dilutions should be determined by each laboratory for each application.
Gln31-Lys372
Analysis
Background: Wnt-5b
Wnt proteins are cysteine-rich secreted glycoproteins that play critical roles in both carcinogenesis and embryonic development. Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase (1-3). Downstream effects of Wnt signaling occur through multiple pathways with differing intracellular components: the canonical Wnt/ beta -catenin pathway, the Wnt/Ca2+ pathway, and the planar cell polarity (PCP) pathway (1-4). Wnt-5b is a 49 kDa glycoprotein that is implicated in the Wnt/Ca2+ and PCP pathways (4‑8). These pathways can inhibit canonical Wnt/ beta -catenin signaling (4, 7). Human and mouse Wnt-5b are synthesized as 359 amino acid (aa) precursors that contain a 17 aa signal sequence and a 342 aa mature region. Mature mouse Wnt-5b shares 94%, 98%, 90% and 88% aa identity with mature human, rat, chick and Xenopus Wnt-5b, respectively. Although Wnt-5a and Wnt-5b share 83% aa identity, their effects may be different. For example, Wnt-5b, but not Wnt-5a, promotes cell cycle progression and is weakly transforming (8, 9). Wnt-5a and -5b are thought to coordinate developmental events, such as chondrocyte differentiation and formation of endochondral bone (5, 6, 10). In contrast to more focused expression of Wnt-5a, Wnt-5b is constitutively expressed at low levels throughout mouse embryonic development (10, 11). In adult mice, Wnt-5b is widely expressed, including heart, liver, brain, lung, testes, kidney, and pancreas (11-13). Wnt-5b is up‑regulated during early adipogenesis, and its overexpression in 3T3-L1 cells partially inhibits canonical Wnt suppression of adipogenesis (7, 14). It enhances PPAR gamma expression and promotes differentiation of preadipocytes (14). Human Wnt-5b polymorphisms have been associated with Type II diabetes (12).
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