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mWnt-8b MAb (Cl 3415 (25 ug)
mWnt-8b MAb (Cl 3415 (25 ug)

mWnt-8b MAb (Cl 3415 (25 ug)

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品牌： R&D Systems
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    来源宿主：
    Rat IgG2a
    Rat IgG2a
    实验应用：
    Western Blot(5 µg/mL)
    Western Blot(5 µg/mL)
    产品介绍
    产品信息
    耦联标记
    Unconjugated
    纯化方式
    Protein A or G purified from hybridoma culture supernatant
    抗原名称
    protein Wnt-8b， wingless-type MMTV integration site family, member 8B， Wnt8b， Wnt-8b
    宿主
    Rat IgG2a
    免疫原
    E. coli-derived recombinant mouse Wnt‑8b
    Trp22-Glu52, Leu195-Arg269
    Accession # Q9WUD6
    克隆号
    341502

    Scientific Data

    Western Blot Detection of Mouse Wnt‑8b antibody by Western Blot.View Larger

    Detection of Mouse Wnt‑8b by Western Blot. Western blot shows lysates of KATO-III human gastric carcinoma cell line. PVDF membrane was probed with 5 µg/mL Rat Anti-Mouse Wnt-8b Monoclonal Antibody (Catalog # MAB33671) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant mouse Wnt-8b (20 ng) was included. A specific band for native Wnt-8b was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.

    Mouse Wnt-8b Antibody Summary

    Species Reactivity
    Mouse
    Specificity
    Detects mouse Wnt-8b in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Wnt-2, -7a, -7b,
    -9a, recombinant mouse Wnt-1, -3a, -4, -5a, -5b, -8a, or -9b is observed.
    Source
    Monoclonal Rat IgG2A Clone # 341502
    Purification
    Protein A or G purified from hybridoma culture supernatant
    Immunogen
    E. coli-derived recombinant mouse Wnt‑8b
    Trp22-Glu52, Leu195-Arg269
    Accession # Q9WUD6
    Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

    Applications

    Recommended Concentration
    Sample
    Western Blot
    5 µg/mL
    See below

    Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

    应用
    实验应用
    Western Blot(5 µg/mL)
    背景
    别名
    protein Wnt-8b,wingless-type MMTV integration site family, member 8B,Wnt8b,Wnt-8b,Wingless-type MMTV Integration Site Family, Member 8b
    背景

    Background: Wnt-8b

    Mouse Wnt-8b is a 40-50 kDa secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329 aa in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt-8b. It appears to play a role in forebrain development. The mature mouse protein is 100%, 98%, and 97% identical to mature rat, canine, and human Wnt-8b, respectively.

    Long Name
    Wingless-type MMTV Integration Site Family, Member 8b
    Entrez Gene IDs
    7479 (Human); 22423 (Mouse); 293990 (Rat)
    Alternate Names
    protein Wnt-8b; wingless-type MMTV integration site family, member 8B; Wnt8b; Wnt-8b
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 0.5 mg/mL in sterile PBS.
    保存方式

    Preparation and Storage

    Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Loading...
    Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
    Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    参考图片

    Detection of Mouse Wnt‑8b by Western Blot. Western blot shows lysates of KATO-III human gastric carcinoma cell line. PVDF membrane was probed with 5 µg/mL Rat Anti-Mouse Wnt‑8b Monoclonal Antibody (Catalog # MAB33671) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant mouse Wnt-8b (20 ng) was included. A specific band for native Wnt-8b was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.

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    货号：
    MAB33671-SP
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    询价
    25ug
    100ug
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