Wnt/beta-Catenin Activated Targets Antibody Sampler Kit

The Wnt family includes several secreted glycoproteins that play important roles in animal development. β-catenin is a key downstream effector of the Wnt signaling pathway that research studies have shown is implicated in early embryonic development and tumorigenesis in vertebrates (1-3). Following binding of Wnt family proteins to the Frizzled receptor, β-catenin translocates to the nucleus where it interacts with LEF1 and TCF1 to activate canonical targets (4). Accepted canonical targets include CD44, cyclin D1, c-Jun, c-Myc, Met, and MMP-7 (5-11). 1.Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305. 2.Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88. 3.Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21. 4.Brantjes, H. et al. (2002) Biol Chem 383, 255-61. 5.Zeilstra, J. et al. (2008) Cancer Res 68, 3655-61. 6.Shtutman, M. et al. (1999) Proc Natl Acad Sci U S A 96, 5522-7. 7.Mann, B. et al. (1999) Proc Natl Acad Sci U S A 96, 1603-8. 8.Wilkins, J.A. and Sansom, O.J. (2008) Cancer Res 68, 4963-6. 9.Boon, E.M. et al. (2002) Cancer Res 62, 5126-8. 10.Brabletz, T. et al. (1999) Am J Pathol 155, 1033-8. 11.Clevers, H. and Nusse, R. (2012) Cell 149, 1192-205.