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YAP (D8H1X) XP ® Rabbit mAb
YAP (D8H1X) XP ® Rabbit mAb
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YAP (D8H1X) XP ® Rabbit mAb

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品牌： CST
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    分子量：
    65-78
    65-78
    反应种属：
    Human,Mouse,Rat,Hamster,Monkey,
    Human,Mouse,Rat,Hamster,Monkey,
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    产品介绍
    产品信息
    抗原名称
    YAP
    来源纯化
    使用对人 YAP 蛋白的羧基末端具有特异性的重组蛋白，对动物进行免疫接种来产生单克隆抗体。表位对应人 YAP 同工型 1 的 Pro435 周围的区域。在人 YAP 蛋白的所有已知同工型中，该序列区域具有 100% 的保守性。
    宿主
    Rabbit
    简单描述
    Monoclonal Antibody for studying YAP (YAP1-2gamma). Cited in 787 publications. Validated for WB, WB, IP, IHC, IHC, IF, IF, F, ChIP, ChIP, C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, YAP (D8H1X) XP® Rabbit Monoclonal Antibody (CST #14074) is ready to ship.
    商品描述

    Product Usage Information

    For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.

    ApplicationDilution
    Western Blotting1:1000
    Simple Western™1:10 - 1:50
    Immunoprecipitation1:50
    IHC Leica Bond1:100 - 1:400
    Immunohistochemistry (Paraffin)1:200 - 1:800
    Immunofluorescence (Frozen)1:50 - 1:200
    Immunofluorescence (Immunocytochemistry)1:50 - 1:200
    Flow Cytometry (Fixed/Permeabilized)1:50 - 1:200
    Chromatin IP1:50
    Chromatin IP-seq1:50
    CUT&RUN1:50
    分子量
    65-78
    内毒素水平
    牛 , 马, 豚鼠
    研究领域
    癌症,发育生物学与干细胞研究,纤维化,神经科学,
    应用
    反应种属
    Human,Mouse,Rat,Hamster,Monkey,
    预测反应种属
    Bovine,Horse,Guinea Pig,
    目标/特异性

    Specificity/Sensitivity

    YAP (D8H1X) XP® Rabbit mAb 可识别内源水平的总 YAP 蛋白。Non-specific labeling of membrane within fixed frozen mouse small intestine may be observed by immunofluorescence.

    物种反应性：

    人, 小鼠, 大鼠, 仓鼠 , 猴

    敏感性
    Endogenous
    背景
    背景

    YAP（Yes 相关蛋白，YAP65）的首次鉴定基于其与 Yes 的 SH3 域结合的能力。它还结合其他包含 SH3 结构域的蛋白，如 Nck、Crk、Src 和 Abl (1)。除了 SH3 结合基序，YAP 还有一个 PDZ 互作基序、一个卷曲螺旋结构域和许多 WW 结构域 (2-4)。YAP 的初步研究都指出，它在锚定和靶向特定亚细胞区室中发挥作用。后续研究表明，YAP 是一个转录共激活蛋白，因为其 WW 结构域与转录因子 PEBP2 和其他转录因子的 PY 基序 (PPxY) 相互作用 (5)。作为一个转录共激活蛋白，YAP 现在被广泛认为是 Hippo 通路的一个中央介导物，从而在组织生长和器官大小的调节中发挥功能性和广泛保守的作用 (6-8)。LATS 激酶磷酸化许多位点（如 Ser109 和 Ser127）会促进 YAP 从细胞核转位到细胞浆，从而通过结合 14-3-3 蛋白被隔离 (7-9)。这些 LATS 诱导的磷酸化活动能让 YAP 进一步被邻近磷酸降解决定子中的 CK1δ/ε 磷酸化，从而诱发 YAP 蛋白酶体降解 (10)。 1.Sudol, M. (1994) Oncogene 9, 2145-52. 2.Mohler, P.J. et al. (1999) J Cell Biol 147, 879-90. 3.Espanel, X. and Sudol, M. (2001) J Biol Chem 276, 14514-23. 4.Sudol, M. et al. (1995) FEBS Lett 369, 67-71. 5.Yagi, R. et al. (1999) EMBO J 18, 2551-62. 6.Dong, J. et al. (2007) Cell 130, 1120-33. 7.Zhao, B. et al. (2010) Genes Dev 24, 862-74. 8.Zhao, B. et al. (2007) Genes Dev 21, 2747-61. 9.Yu, F.X. et al. (2012) Cell 150, 780-91. 10.Zhao, B. et al. (2010) Genes Dev 24, 72-85.

    研究领域
    癌症,发育生物学与干细胞研究,纤维化,神经科学,
    翻译后修饰
    Unmodified
    制备和贮存
    保存方式
    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #53921.
    数据库链接
    Entrez-Gene ID
    10413
    UniProt ID
    P46937

    参考图片

    Western blot analysis of extracts from various cell lines using YAP (D8H1X) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, RL-7 cells are negative for YAP protein expression.

    Confocal immunofluorescent analysis of low confluence MCF 10A cells (left), high confluence MCF 10A (center), and YAP negative RL-7 cells (right) using YAP (D8H1X) XP® Rabbit mAb (green). Blue pseudocolor in lower images = DRAQ5® #4084 (fluorescent DNA dye). Increased nuclear localization of YAP protein is seen in low confluence (proliferating) cells.

    Immunohistochemical analysis of paraffin-embedded PANC-1 (left) and RL-7 (right) cell pellets using YAP (D8H1X) XP® Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma using YAP (D8H1X) XP® Rabbit mAb.

    Immunoprecipitation of YAP protein from A-204 cell extracts using Rabbit (DA1E) mAb XP® Isotype Control #3900 (lane 2) or YAP (D8H1X) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using YAP (D8H1X) XP® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody.

    Flow cytometric analysis of RL-7 cells (blue) and A-204 cells (green) using YAP (D8H1X) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. 

    Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma using YAP (D8H1X) XP® Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using YAP (D8H1X) XP® Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded human benign prostatic hyperplasia using YAP (D8H1X) XP® Rabbit mAb.

    Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCI-2052 cells and either 10 µl of YAP (D8H1X) XP® Rabbit mAb, or 2 µl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CTGF Promoter Primers #14927, human SMYD3 intron 2 primers, and SimpleChIP® Human CTGF Upstream Primers #14928. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

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    货号：
    14074T
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